Figure 5.

Immunoblot analysis of diagnostic components of the multiprotein complexes in the thylakoid membrane in transplastomic Δycf4 knockout mutants, wild-type plants (WT), and KD-psaA control plants grown under autotrophic conditions. Isolated thylakoid proteins were separated by SDS-PAGE, blotted, and probed with antibodies against the proteins indicated on the left side of each panel. Equal amounts of chlorophyll were loaded. For quantitative assessment of protein accumulation in the mutants, a dilution series of the wild-type sample (100%, 50%, and 25%) was loaded. A Ycf4-specific antibody generated in this study was used to assess the accumulation of the ycf4 gene product.