Table II. Regeneration of Tpx or MSR family members by wild-type and mutated Trx-like2.1 in the presence of an NADPH/NTR system (NTS) or an NADPH/GR/GSH system (NGS).
Data are expressed as nmol oxidized NADPH min−1 nmol−1 enzyme. Dashes indicate that no significant activity was detected.
| Targets | Trx h1 |
Trx-like2.1 |
Trx-like2.1 C45S |
Trx like2.1 R43G/K44P |
||||
| NTS | NGS | NTS | NGS | NTS | NGS | NTS | NGS | |
| PtPrxIIB | 7.7 ± 0.5 | –- | –- | 16.3 ± 1.1 | –- | 15.7 ± 0.1 | 5.8 ± 0.2 | –- |
| PtPrxIIE | 11.6 ± 1.5 | –- | –- | 2.4 ± 0.4 | –- | 7.8 ± 0.3 | 8.4 ± 0.3 | –- |
| PtPrxQ | 10.7 ± 0.5 | –- | –- | –- | –- | –- | 2.4 ± 0.4 | –- |
| PtGpx1 | 10.2 ± 0.8 | –- | –- | –- | –- | –- | 4.8 ± 0.7 | –- |
| PtGpx3 | 10.2 ± 0.2 | –- | –- | –- | –- | –- | 5.6 ± 0.1 | –- |
| PtMSRA4 | 2.6 ± 0.2 | –- | –- | –- | –- | –- | 1.6 ± 0.3 | –- |
| AtMSRB2 | 16.2 ± 1 | – | –- | –- | –- | –- | –- | –- |
| AtMSRB1 | –- | –- | –- | 1.9 ± 0.1 | –- | 4.4 ± 0.1 | –- | –- |