Figure 2.
BFA compartments of ABCB4 colocalized with SNX1. A and B, Colocalization of ABCB4-GFP and FM4-64 in the BFA compartment. Transgenic seedlings were treated with 50 μm BFA for 2 h and then cotreated with 2 μm FM4-64 for 15 min (B). The same volume of DMSO was administered as a control (A). C, Colocalization analysis of ABCB4-GFP and SNX1-mRFP with BFA (50 μm, 2 h). D, Colocalization analysis of ABCB4-GFP and mRFP-ARA7 with BFA (50 μm, 2 h). E, Colocalization analysis of ABCB4-GFP and YFP-RHA1 with BFA (50 μm, 2 h). F, Colocalization analysis of ABCB4-GFP and ST-RFP with BFA (50 μm, 2 h). Bars + 10 μm.