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. 2012 Apr 17;159(2):655–670. doi: 10.1104/pp.111.192682

Figure 4.

Figure 4.

Light micrographs of silver-enhanced (1→3, 1→4)-β-d-glucan labeling of differentiating endosperm from 10 to 22 DAP. A, At 10 DAP (1→3, 1→4)-β-d-glucan labeling was present in all cell walls of the differentiating endosperm. B, At 12 DAP the (1→3, 1→4)-β-d-glucan monoclonal antibody labeled the cell walls of the differentiating aleurone and central starchy endosperm. Labeling was absent from the cell walls of the peripheral endosperm. C, At 16 DAP (1→3, 1→4)-β-d-glucan was present in the cell walls of the peripheral endosperm. D, Increased labeling intensity implies (1→3, 1→4)-β-d-glucan is in greater abundance in the starchy endosperm cell walls than either the aleurone or maternal tissues. Bars = 100 μm. al, Aleurone; cse, central starchy endosperm; mt, maternal tissues; pe, peripheral endosperm; se, starchy endosperm; sa, subaleurone.