Table I. Gene-specific QPCR primers, with PCR product sizes in bp and optimal acquisition temperatures for the genes analyzed.
| Name | Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) | PCR Product | Acquisition Temperature | Accession No. |
|---|---|---|---|---|---|
| bp | °C | ||||
| HvCSLA1 | CGTTTCTTTTCTTCTGTGCGTCAT | GAAACTGTATAACAGCACTTGGTA | 175 | 76 | AK359952.1 |
| HvCSLA2 | GCGTTCGGGAAGGACAAT | GAGAAGACTGCGTGATCTTTACT | 215 | 80 | AK371878.1 |
| HvCSLA3 | GTATGATTACGTGCTTGGAA | TATGGATTGCCGTTGTCAGAT | 199 | 79 | AK375227.1 |
| HvCSLA4 | TTCCACTCCCAGTGCCGAATAA | GCAGCAAAGAATGGTTGGATCA | 171 | 78 | AK367304.1 |
| HvCSLA5 | GCTCGTGCAGATCCCCATGT | AAGGAAGCTAGATTCAGGTTGGAAAT | 319 | 83 | AK372922.1 |
| HvCSLA6 | CTGCCGCTGCTTCTGCTACC | GCCGAATCCGATGACGAATG | 236 | 84 | AK376872.1 |
| HvGT61 | CCAACCAGCCCAGGTAAAC | CTTTCCAGCAGCAGTAGTAGTGA | 126 | 78 | AK253054.1 |
| HvGT43 | GCTGGTCTGATCTGTGTGAG | GATGTATCGTCTGTGACTTTCT | 124 | 79 | AJ969053.1 |
| HvGT47 | TAGCATCTCTCGCCTCGC | TTGACTTATGGTCAGCACTGTG | 125 | 77 | AK366860.1 |