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. 2012 Apr 23;159(2):671–681. doi: 10.1104/pp.111.191510

Figure 2.

Figure 2.

SDS/PAGE and IEF/SDS-PAGE analysis of the chromatography-purified PTPase. A, The protein fractions from the chromatography column were run with a 12% acrylamide monomer concentration. Key: M, Molecular weight marker; 1, crude extract; 2, DEAE-Sepharose chromatography; 3, SP Sepharose F.F. chromatography; 4, Sephacryl S-200 chromatography; 5, Sephacryl S-300 chromatography. B, The final protein fraction eluted through a Sephacryl S-300 chromatography column was subjected to IEF/SDS-PAGE with a 12% acrylamide monomer concentration and the protein was stained with silver.