Figure 4.

At4g15400 encodes a BAHD acyltransferase-like protein. A, Phylogenetic tree based on the predicted amino acid sequences of At4g15400 (red) and all other BAHD acyltransferases in Arabidopsis. Clade IV does not exist in Arabidopsis. Amino acids were aligned using the Clustal W program and a phylogenetic tree was constructed using MEGA 5.05 software by scoring the number of amino acid differences (pairwise distance). Scale bar indicates the number of amino acid differences. B, Comparison of highly conserved motifs in BIA1 and other proteins in clade III of the BAHD family in Arabidopsis. Bold characters in the squares represent two conserved motifs. Amino acids of At4g15400 that differ from those of other BAHD family proteins are shaded. Red font indicates conserved motifs in BIA1.