Figure 9.

Altered disease resistance of transgenic VQ-overexpressing Arabidopsis plants. A, Enhanced susceptibility to B. cinerea. Col-0 wild type (WT) and two independent lines (L) of transgenic plants overexpressing VQ5 or VQ20 were spray inoculated with Botrytis and the picture was taken at the third d post inoculation (dpi). B, Enhanced susceptibility to P. syringae. Col-0 wild type (WT) and transgenic plants overexpressing VQ20 (lines 12 and 13) or VQ25 (lines 7 and 8) were inoculated with a virulent strain of P. syringae pv tomato DC3000 (OD₆₀₀= 0.0002 in 10 mm MgCl₂). Samples were taken at 0 and 3 dpi to determine the growth of the bacterial pathogen (top section). According to Duncan’s multiple range test (P= 0.01), means of colony-forming units (cfu) do not differ significantly at 0 dpi if they are indicated with the same lowercase letter and do not differ significantly at 2 dpi if they are indicated with the same uppercase letter. Images from representative inoculated leaves taken at 3 dpi are also shown (bottom section). The RNA-blotting analysis of the overexpressed VQ genes in the transgenic lines is shown in Supplemental Figure S2S.