Figure 6.

Analysis of light-induced phosphorylation of the H⁺-ATPase in thalli. A, Effects of light quality on the phosphorylation of the H⁺-ATPase. Dark-adapted thalli were illuminated with red light (RL), blue light (BL), or white light (WL) for 30 min at 50 µmol m⁻² s⁻¹. The protein extracts were subjected to SDS-PAGE. Other procedures were the same as in Figure 4A. B and C, Effects of DCMU (B) and DBMIB (C) on light-induced phosphorylation of H⁺-ATPase. Dark-adapted thalli were incubated with (+) or without (−) 10 µm DCMU or 10 µm DBMIB for 30 min in the dark. Thalli were then illuminated with white light (Lt) at 50 µmol m⁻² s⁻¹ or kept in the dark (Dk) for 30 min. Other procedures were the same as in Figure 4A.