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Immunoblot of VIP1-GFP. Plants were transformed with a construct containing CaMV 35S promoter-VIP1-GFP (VIP1-GFPox #2 and #3), and 10-d-old seedlings were subjected to immunoblot analysis using anti-GFP antibody. Wild-type plants (WT) and transgenic plants expressing GFP alone (GFPox) were used as controls. The arrowhead designates a nonspecific band, which served as a loading control.
