Figure 4.

A transient reporter assay for CYP707A1/3 promoter-VIP1 interactions in plant cells. A reporter construct containing CYP707A1/3 promoter-luciferase (CYP707A1/3pro-LUC) was introduced into Arabidopsis mesophyll protoplasts with an effector construct to express GFP or VIP1-GFP. Reporter luciferase (firefly luciferase) activities were standardized by activities of coexpressed Metridium luciferase, and relative reporter activities were calculated. Experiments were repeated three or four times for each reporter-effector combination. Values are means ± se.