Figure 5.

Relationship between Phi effectiveness and ROS, camalexin, and scopoletin accumulation. Plants were treated and inoculated as described in the legend of Figure 3. A, Phi effectiveness in Col-0 wild-type plants (WT) and atrbohD, pad3-1, and f6′h1-1 mutants. Phi effectiveness was calculated as described in the legend of Figure 2. B, Levels of camalexin. C, Levels of total scopoletin. Values are means ± se of 15 replicates from five biological independent experiments in A and means ± sd of three replicates in B and C. No significant differences were found between values in A, B, and C (ANOVA, Newman-Keuls test; P < 0.05). The experiments in B and C were repeated twice with similar results. Means ± sd of spore numbers quantified on mock-pretreated plants of Col-0 wild-type plants and atrbohD, pad3-1, and f6′h1-1 mutants were 610 ± 75, 575 ± 46, 615 ± 40, and 680 ± 48 spores mg⁻¹ fresh weight (FW), respectively.