Figure 2.

Targeted YTH assay of HvRBK1 and barley HvROPs in yeast. Targeted YTH assay in yeast strain AH109 cotransformed with HvRBK1 in the pGADT7 prey vector and barley HvROPs in pGBKT7 bait vector. Bait and prey interaction allows growth on selective synthetic dextrose media without −Leu, −Trp, −His, and adenine (−Ade). No growth was observed on selective media when yeast was cotransformed with expression constructs of HvROPs and the empty vector pGADT7 or with HvRBK1 and empty vector pGBKT7. As control for successful cotransformation, yeast cells were dropped on selective synthetic dextrose media −Leu, −Trp (bottom picture).