Table I. Efficiency of FRET between HvRBK1-YFP and variants of CFP-HvRACB.
Quantitative analysis of acceptor photobleaching FRET measurements. Data show the means of three independent experiments. Seven to 10 cells were analyzed in each experiment. FRET efficiency was significantly elevated in cells transformed with HvRBK1-YFP and CFP-CA HvRACB compared with cells cotransformed with HvRBK1-YFP and CFP-DN HvRACB (two-tailed Student’s t test; ***, P > 0.001). Errors represent the sds.