Figure 5.
Overexpression of miR396 and the target genes GRF1 and GRF3 negatively impacts root development. A and B, Transgenic plants overexpressing miR396a (line 22-5; A) or miR396b (line 15-1; B) develop shorter roots than the wild type (Col-0). C, Transgenic plants overexpressing rGRF1 (line 18-2) or rGRF3 (line 11-15) develop shorter roots than the wild type (Col-0). Homozygous T3 plants were planted on modified Knop’s medium along with the wild type, and 10-d-old seedlings were photographed. Root length measurements are provided in Supplemental Figure S6.