Table I. Phosphorylation motifs identified in Arabidopsis roots.
The phosphorylated residue is indicated in lowercase underlined letters (s for Ser and t for Thr), and X denotes any amino acid. For sequence alignment, see Supplemental Figure S1.
| No. | Motif | Motif Score | No. | Fe Responsive | No. of Fe-Responsive Peptides | Putative Kinasea |
|---|---|---|---|---|---|---|
| I | ||||||
| 1 | E.....sP..... | 21.21 | 21 | Yes | 2 | Pro-directed kinase |
| 2 | .E....sP..... | 19.84 | 16 | No | ||
| 3 | ...... sP..... | 16.00 | 93 | Yes | 12 | |
| II | ||||||
| 4 | ...... sD.E... | 28.07 | 27 | No | CK II | |
| 5 | ...... sEDE... | 21.89 | 7 | No | ||
| 6 | ...... s.E.EE. | 16.73 | 8 | No | ||
| 7 | ...... sDDD... | 15.18 | 7 | Yes | 2 | |
| 8 | ...... s.E.... | 3.95 | 15 | No | ||
| 9 | .....As.E.... | 9.22 | 8 | No | ||
| 10 | ...... s.KE... | 9.65 | 9 | Yes | 1 | |
| 11 | .....Ds.D.... | 8.24 | 7 | No | ||
| III | ||||||
| 12 | ...H.. s.S.... | 10.82 | 8 | Yes | 1 | GSK3-like kinase |
| 13 | ....S. s...... | 4.06 | 26 | Yes | 1 | |
| IV | ||||||
| 14 | ...... s.S.... | 3.34 | 30 | Yes | 2 | PKA |
| V | ||||||
| 15 | ...... s.H.... | 3.56 | 8 | Yes | 2 | Protein kinase C |
| 16 | ...KS. s..N... | 14.43 | 6 | Yes | 1 | |
| VI | ||||||
| 17 | .....Gs...... | 4.51 | 27 | Yes | 2 | Novel CK II |
| VII | ||||||
| 18 | ...... s.....X | 16.00 | 7 | Yes | 1 | Unknown |
| VIII | ||||||
| 19 | ...R.. s...... | 16.00 | 74 | Yes | 5 | PKA/calcium calmodulin-dependent kinase II |
| IX | ||||||
| 20 | ......tD..... | 3.36 | 10 | Yes | 2 | CK II |
| X | ||||||
| 21 | ......tP..... | 3.55 | 10 | Yes | 2 | Pro-directed kinase |
a
Predicted by NetPhosK 1.0 (http://www.cbs.dtu.dk/services/NetPhosK/). The putative kinase with the highest score was chosen.