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. 2012 Mar 5;159(1):450–460. doi: 10.1104/pp.112.195578

Figure 1.

Figure 1.

PLDα1 and PLDδ expression and the effect of mutation of PLDs in ABA-induced stomatal closure and PA production. A, RT-PCR analysis of PLDα1 and PLDδ gene expression in wild-type GCPs and MCPs. ACTIN2 was used as a positive control for the RT-PCR. KAT1 was used as a control of GCP gene expression. B, The positions of T-DNA insertions in pldα1 and pldδ mutants. Boxes represent exons. C, RT-PCR analysis of PLDα1 and PLDδ gene expression in the wild type (WT) and pldα1, pldδ, and pldα1 pldδ mutants. ACTIN2 was used as a positive control for the RT-PCR. D, ABA-induced stomatal closure in the wild type and pldα1, pldδ, and pldα1 pldδ mutants. Averages from three independent experiments (60 stomata per bar) are shown. E, Drought-induced PA accumulation in rosette leaves of the wild type and pld mutants. F, ABA-induced PA accumulation in epidermal tissues of the wild type and pld mutants. Radioactivity was normalized to the control values. Averages from six independent experiments are shown. Error bars represent se.