Figure 3.

Effects of the PLD inhibitor 1-BuOH on ABA-induced stomata closure and PA in the wild type (WT) and pld mutants. A, Rosette leaves of the wild type and pld mutants were treated with 10 μm ABA and with or without 50 μm 1-BuOH. 1-BuOH was applied to the assay solution before ABA treatment. B, PA induced stomatal closure in pld mutants. Rosette leaves of the wild type and pldα1, pldδ, and pldα1 pldδ mutants were treated with 10 μm ABA, 10 μm PA, and 50 μm PA. Averages from three independent experiments (60 stomata per bar) are shown. Error bars represent se.