Figure 4.

Expression of MtROP9 and other related genes in MtROP9i and Mtvector transgenic roots after A. euteiches (Ae), G. intraradices (Gi), and S. meliloti (Sm) infection at 3 and 24 hpi. For quantitative evaluation of relative in-gel intensities of the amplified PCR products, intensities were calculated relative to the values for the EF1α reference gene (first two bars of each graph; scaled as 1). The data shown represent averages and se of five biological replicates. Expression rates for MtROP9i are presented as orange bars and those for Mtvector as green bars. Asterisks indicate levels of significance by Student’s t test on all expression values evaluated for Mtvector and MtROP9i: *** P < 0.001, ** P < 0.01, * P < 0.05. [See online article for color version of this figure.]