Figure 2.

Absence of primed defense gene activation in hsfb1 mutants. Five-week-old wild-type (WT), hsfb1-1, and hsfb1-3 plants were grown as described in Figure 1 and treated with 100 µm BTH or the blank formulation for 3 d. Then, leaves were either left untreated (−) or infiltrated with water (+). Three hours later, leaves were assayed for the accumulation of PAL1 (A) or WRKY29 (B) transcripts by qRT-PCR. Relative amounts of gene expression were normalized to those of ACTIN2. Error bars indicate sd (n = 3). The experiment was repeated three times with similar results.