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. Author manuscript; available in PMC: 2013 Jun 1.

Published in final edited form as: J Cosmet Dermatol. 2012 Jun;11(2):122–130. doi: 10.1111/j.1473-2165.2012.00606.x

(A) Increase in dermal collagen content after Er:YAG 2940 nm laser mini-peels. Immunohistochemical staining of skin tissues for collagen types I and III (1^st and 2^nd rows, respectively) showing an increase in collagen content. Shown in the 3^rd and 4^th rows are representative examples of skin tissues stained with picrosirius red viewed under bright field (3^rd row) and polarized field (4^th row). Bright field captures total collagen content while polarized light showed yellow to orange birefringence reflecting newly synthesized collagen in yellow to orange and total collagen in red. An increase in collagen type VII expression (red; 5^th row) was observed after Er:YAG mini-peels compared to base line, and then decreased 3 months post treatment. Nuclei stained in blue with DAPI (Immunohistochemical and Picrosirius red; X 200); (B) Percent of dermis occupied by collagen levels. Data showed a statistically significant increase in both collagen types I, III and VII, as well as newly synthesized collagen at the end of Er:YAG laser mini-peels (*,p≤ 0.05).

(A) Increase in dermal collagen content after Er:YAG 2940 nm laser mini-peels. Immunohistochemical staining of skin tissues for collagen types I and III (1^st and 2^nd rows, respectively) showing an increase in collagen content. Shown in the 3^rd and 4^th rows are representative examples of skin tissues stained with picrosirius red viewed under bright field (3^rd row) and polarized field (4^th row). Bright field captures total collagen content while polarized light showed yellow to orange birefringence reflecting newly synthesized collagen in yellow to orange and total collagen in red. An increase in collagen type VII expression (red; 5^th row) was observed after Er:YAG mini-peels compared to base line, and then decreased 3 months post treatment. Nuclei stained in blue with DAPI (Immunohistochemical and Picrosirius red; X 200); (B) Percent of dermis occupied by collagen levels. Data showed a statistically significant increase in both collagen types I, III and VII, as well as newly synthesized collagen at the end of Er:YAG laser mini-peels (*,p≤ 0.05).