Figure 3.

Genotyping for rd8. (A) Genotyping of vendor mice by PCR for rd8. Bands were resolved by capillary electrophoresis. Mice are from HSD, DCT, CRL, TAC, and JAX. Genotyping for rd8 was performed by PCR. Two representative mice out of 7 obtained from each vendor in 2 separate shipments are shown. (B) Sequencing of Crb1 gene shows a single base deletion in the N substrain at the expected position in the aligned sequences (MegAlign program). N-MF, N substrain sequenced with forward primer; N-MR, N substrain sequenced with reverse primer; J-MF, J substrain sequenced with forward primer; J-MR, J substrain sequenced with reverse primer.