Figure 4.

Propranolol fails to suppress OIR and does not affect angiogenic factors in OIR. (a) Representative images of retinal whole mounts isolated from P17 mice with induced retinopathy treated with propranolol (SC) or vehicle control three times per day from P12 to P16 (60 mg/kg/day). Retinas were stained with isolectin B₄ (red) to visualize vasculature. Quantifications of VO and NV are highlighted. Scale bar: 1000 μm. (b) Quantification of VO and (c) NV as percentage of total retinal area. (d) Retinal expression of angiogenic factors (Vegf, Epo, Ang1, and Ang2) in OIR mice treated with propranolol (SC, 60 mg/kg/day) or control were quantified with RT-qPCR. (e) Protein levels of retinal VEGF from OIR mice treated with propranolol (SC, 60 mg/kg/day) or control were measured with ELISA assay. N.S., not significant.