Figure 3.

Presence of histamine receptors in rat conjunctiva and cultured goblet cells. Cultured rat goblet cells were homogenized, and RNA isolated for RT-PCR with primers for histamine receptors (A). The blot is representative of three individual animals. Rat conjunctiva and cultured goblet cells were homogenized and Western blot analysis was performed with anti-histamine receptor subtype antibodies, shown in (B). The blot is representative of three individual animals. MWM, molecular weight markers; H₁R, histamine receptor type 1; H₂R, histamine receptor type 2; H₃R, histamine receptor type 3; H₄R, histamine receptor type 4; NC, negative control; Gb, cultured goblet cells; Conj, rat conjunctiva.