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. 2012 May 17;53(6):2993–3003. doi: 10.1167/iovs.11-8748

Table.

Primers for RT-PCR

Receptor	Primer Sequences 5′-3′		Annealing Temp (deg C)	Fragment Size (bp)	Source
HR₁	Sense:	`CCTCTACCTTCGAAGACAAG`	60	406	NM_017018
HR₁	Anti:	`GACCAAAGAGATGGCAAC-`	60	406	NM_017018
HR₂	Sense:	`ATGGCATTGAAAGTCACC`	60	378	NM_012965.3
HR₂	Anti:	`GACCAAAGAGATGGCAAC`	60	378	NM_012965.3
HR₃	Sense:	`CAGCGTTACCTTCTTCAACC`	60	512	NM_053506.1
HR₃	Anti:	`CAGCTCGGATGATCATTAGG`
HR₄	Sense:	`TAACGATAGGCAATGCTGTG`	60	391	NM_131909.1
	Anti:	`TCTTCCAAGAATCCGAAGCC`		228
	Nested Anti:	`ACTGTAGACGGATGCTGTGC`
β-actin		`ATGGATGACGATATCGCTG`	59	568	NM_03114.2
β-actin		`ATGAGGTAGTCTGTCAGGT`	59	568	NM_03114.2