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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Int Rev Cytol. 2002;217:137–182. doi: 10.1016/s0074-7696(02)17014-8

FIG. 13.

FIG. 13

Arp2/3 is required for actin polymerization and for shape change in platelets. (A) Pyrene-actin assay showing the effects of anti-Arp2 on actin polymerization in TRAP-stimulated permeabilized platelets. (B) Histogram of the morphological effects of Arp2/3 inhibition on platelets loaded with pre-immune-antibodies (Pre), anti-Arp2 antibody (αArp) or its Fab fragments (Fab). Anti-Arp2 decreases filopodial formation, and freezes platelets at the rounded. Recombinant Arp2 (rArp) blocks this effect. (C) Arp2/3 is located at the tips of filopodia (arrows) and in the lamellipodia in two platelets spread on glass and double labeled for Arp2 (top) and actin (bottom) (Modified from Li, Kim and Bearer, 2002, with permission).