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. 2012 Jun 15;7(6):e39151. doi: 10.1371/journal.pone.0039151

Figure 4. MiR-127 down-regulates Bcl6 and Setd8 expression.

Figure 4

(A and B) BRL-3A (A) and Huh7 (B) cells transfected with miR-127 mimics or miRNA NC were analyzed by qRT-PCR (left) and Western blotting (right), respectively. Actin was used as a control. (C) Graphic overview of the conserved 3′UTR sequences of Setd8 among rat, mouse and human. The “seed sequences” in the 3′UTRs of Setd8 mRNA were indicated (red box). (D) The repression of Setd8 gene mediated by the 3′-UTR was analyzed by luciferase reporter assay. BRL-3A cells were cotransfected with a luciferase reporter vector containing the 3′-UTR or the mutated 3′-UTR (Mu-UTR) of Setd8 and miR-127 mimics (miR-127) or the negative control (NC). The pGL4.13 control vector was used as a control. The wild-type and mutated miR-127-binding sites in the 3′-UTR of this gene were indicated (left). Data from three independent experiments are shown as the means ± SD. (*P<0.05, **P<0.01).