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. 2012 Jun 15;7(6):e39151. doi: 10.1371/journal.pone.0039151

Figure 5. Down-regulation of miR-127 may participate in cell growth regulation through Bcl6 and Setd8 during the first 24 h after PH.

Figure 5

(A) CDKN1A, but not p53, is a downstream target gene of Bcl6. BRL-3A cells were transfected with Bcl6 siRNA or a negative control (siRNA NC), and the relative expression levels of mRNA (left) and protein (right) were analyzed by qRT-PCR and Western blotting, respectively. Actin was used as a loading control. (B) Down-regulation of Bcl6 suppresses hepatocyte proliferation. BRL-3A cells were transfected with Bcl6 siRNA or siRNA NC, and the relative cell proliferation was examined at the indicated time by methylthiazol tetrazolium. (C) MiR-127 decreased the protein expression of monomethyl histone H4. The protein expression of cells treated with miR-127 mimics or miRNA NC was analyzed by Western blotting. Actin was used as a control. (D) The expression of monomethyl histone H4 during LR was analyzed by Western blotting. Actin was used as a control. (E) Liver sections were stained with PCNA (400× magnification) from 3 to 72 h after PH. All the data were obtained from at least three independent experiments and are shown as the means ± SD. (*P<0.05, **P<0.01).