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. 2012 Jun 15;7(6):e39151. doi: 10.1371/journal.pone.0039151

Figure 6. MiR-127 is induced from its promoter by DNA demethylation and histone deacetylase inhibition.

Figure 6

(A) Schematic representation of the CpG island on the promoter region of the miR-127 gene. (B) MiR-127 is highly induced by 5-Aza-CdR and PBA treatment. BRL-3A cells were treated with 5-Aza-CdR and/or PBA, and the miR-127 expression level was analyzed by qRT-PCR. U6 RNA expression was used as a loading control. 0.1A, 0.1 μmol/L 5-Aza-CdR; 1A, 1 μmol/L 5-Aza-CdR; 3A, 3 μmol/L 5-Aza-CdR; 0.1P, 0.1 mmol/L PBA; 1P, 1 mmol/L PBA; 3P, 3 mmol/L PBA; 0.1AP, combination of 0.1 μmol/L 5-Aza-CdR and 0.1 mmol/L PBA; 1AP, combination of 1 μmol/L 5-Aza-CdR and 1 mmol/L PBA; 3AP, combination of 3 μmol/L 5-Aza-CdR and 3 mmol/L PBA. Data from three independent experiments are shown as the means ± SD. (**P<0.01). (C) The CpG island was hypermethylated 24 h after partial hepatectomy (PH). Genomic DNA was obtained from rat liver tissues 24 h after PH or sham operation (SH), the DNA methylation status was determined by bisulfite genomic sequencing (P<0.01, Wilcoxon rank-sum test).