Table 1. Primers Used in qRT-PCR and Vector Construction.
Primer Names | Sequences (5′ > 3′) |
miR-127 | AGAGGCGGACGGTGTCGTAGTTGAAGTGAGCCGTCCGCCTCTAGCCAAGC |
GATGTCGGATCCGTCTGAGC | |
CGGTGTCGTAGTTGAAGTGAG | |
U6 | GTGCCTGCTTCGGCAGCACATA |
TGGAACGCTTCACGAATTTGCGTGTC | |
β-Actin | CACCCGCGAGTACAACCTTC |
CCCATACCCACCATCACACC | |
Human-β-Actin | TGATATCGCCGCGCTCGTCG |
ACCCATGCCCACCATCACGC | |
Bcl6 | TCGAGGTCGTGAGGTTGT |
CGGATAAGAGGCTGGTGG | |
Human-Bcl6 | GAGCCTTTGCCCCCAGCCTG |
GGGGTTGGCCACAGGCATCC | |
Setd8 | ATCACCGATGCCAAGAAGC |
CGTCGATGTCATGCAGTTTG | |
Human-Setd8 | GCAAACTTACGGATTTCTACCCT |
CATTCCTTCTTCCTTCCCACTT | |
p53 | CGGCTCCGACTATACCACTAT |
GGACAGGCACAAACACGAA | |
Human-p53 | GCTTTGAGGTGCGTGTTTGT |
TTGGGCAGTGCTCGCTTAG | |
CDKN1A | GCAAAGTATGCCGTCGTCT |
CAAAGTTCCACCGTTCTCG | |
MSP* | TTTTTGAGTTTTAGTAGGTTGG |
ACCAAACRATACTCTCCATATA | |
Setd8 3′-UTR | GCTCTAGATAACCTGTGATAGCAAGAGTGGG |
GATGGCCGGCCCCACAAACAGGTACAACCAAGAC | |
Setd8-Mu 3′-UTR | TCAGACTTGGAGGAGACGCAGACAGGAGGGTGTAAC |
GTTACACCCTCCTGTCTGCGTCTCCTCCAAGTCTGA |
Primers used for bisulfite genomic sequencing.