Figure 4. Quantification of viral DNA replication in human cancer cells under normoxia or hypoxia.

The indicated human cancer cells were infected with OBP-301 or Ad5 at an MOI of 50 PFU/cell for 1 h, and were further incubated under normoxic (Nx) or hypoxic (Hx) conditions for 48 h. After incubation, cells were harvested and counted. E1A copy number in the cells at 48 h after incubation under normoxia or hypoxia was analyzed by quantitative PCR analysis. The amount of virus production was defined as the value of the E1A copy number relative to the number of cancer cells. Data are shown as the mean values ± SE of triplicate experiments. Statistical significance (*) was determined as P<0.05 (Student's t test).