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. 2012 Jun 15;7(6):e39063. doi: 10.1371/journal.pone.0039063

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Mader, Kunert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Three different Ab2/3H6^Fab preparations were developed for the immunization study. Panel A. displays (a) the Fab-fragment HC of Ab2/3H6 named Ab2/3H6^Fab. (b) human IL15 fused to Ab2/3H6^Fab named Ab2/3H6^Fab-IL15 and (c) a tetanus toxin epitope fused to Ab2/3H6^Fab named Ab2/3H6^Fab-TT. The chimeric mouse 3H6vL/hukappa LC (214 aa) is not shown. Panel B. shows the non-reduced SDS-gel of purified Fabs; silver stain (SS) on the left side and the Western Blot (WB) on the right side developed using an anti-human Fab specific antibody and visualized with NBT/BCIP. The lanes 1 represents Ab2/3H6^Fab-TT (49 kD); lane 2: Ab2/3H6^Fab-IL15 (61 kD); lane 3: Ab2/3H6^Fab (47 kD) and M represents the marker. The double band is significant for glycosylated Ab2/3H6 [61].