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. 2012 Jun 15;7(6):e38053. doi: 10.1371/journal.pone.0038053

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Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunoblotting analysis of Nkx2.5 and O-GlycNAcylated proteins in the heart tissue of DM intraperitoneally injected with streptozotocine (180 mg/kg body weight). (B) Graphs showed relative O-GlcNAc (left) and Nkx2.5 (right) levels normalized to the tubulin levels in DM and control. Nkx2.5 protein in diabetic heart of mice was significantly decreased. O-GlcNAcylation and Nkx2.5 protein levels were compared in DM vs control. All values are presented as the mean±standard error of three independent experiments. *p<0.05 DM vs. control.