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. 2012 Jun 15;7(6):e39065. doi: 10.1371/journal.pone.0039065

Figure 4. WFA treatment induces an increase in the phosphorylation of vimentin serine-38.

Figure 4

BJ-5ta cells were treated for 3 hrs with DMSO (A) or 2 μM (B) WFA, then fixed and double labeled with vimentin (A′ and B′) and pSer38 vimentin (A′′ and B′′) antibodies. Scale bars =10 μm. Arrow: a region depicted at higher magnification in the inset showing vimentin particles stained with pSer38 vimentin antibody. (C) Whole cell lysates of cells treated with DMSO (ctrl) or 2 μM WFA for 60 min, 120 min, and 180 min, were separated by SDS-PAGE and stained with anti-vimentin and anti-vimentin pSer38 vimentin antibodies.