Figure 5. WFA does not affect the sedimentation velocity of vimentin.

Sedimentation velocity profile of vimentin (0.15 mg/ml) reconstituted in 5 mM Tris-HCl, pH 8.4 (dashed lines) or 2 mM NaCl, pH 7.5 buffer (solid lines) alone (red lines), with DMSO (green lines), or with 25 μM WFA dissolved in DMSO (blue lines). Note that the curves of the two groups of runs are practically superimposable indicating that DMSO and DMSO plus WFA do not have any effect on the complex formation of vimentin oligomers.