Figure 7. WFA affects the organization of keratin and neuron specific IF.

MCF-7 cells, which express only keratin IF, were treated for 3 hrs with DMSO (A) and 4.0 μM WFA (B; the lowest effective concentration in these cells) and stained with pan-cytokeratin antibodies. Differentiated PC12 cells were treated for 3 hrs with DMSO (C) or 1.0 μM WFA (D, the lowest effective concentration for these cells), then stained with neurofilament-M (C′ and D′) and peripherin antibodies (C′′ and D′′). Scale bars =10 μm.