Figure 2. Expression levels of 8 miRNAs in sepsis non-survivors and survivors in a confirmation set.

These 8 miRNAs were significantly differentially expressed between sepsis non-survivors and survivors after qRT-PCR validation in a smaller study sample. Then, these were checked by qRT-PCR in a larger study sample size (Non-survivors = D, n = 73; Survivors = S, n = 93). Only 6 of the 8 miRNAs remained as significantly different between the D group and S groups. Expression levels of these 8 miRNAs were normalized to U6 snRNA U6 snRNA above normal controls and given as fold-changes (2^–ΔΔCt ), △△Ct =  (Ct_miRNA-Ct_{U6 snRNA})_patients-(Ct_miRNA-Ct_{U6 snRNA})_controls. Mann-Whitney U-test or student t-test was used for statistical comparisons.