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. 2012 Jun 15;7(8):e37168. doi: 10.1371/journal.pone.0037168

Figure 4. Effects of DCO-6 on p38 MAPK activation induced by different TLR ligands in RAW264.7 cells.

Figure 4

(A) Cells were treated with DCO-6 in the absence or presence of indicated TLR ligands for 6 h. Whole cell lysates were prepared for Western blotting analysis. The protein levels of total and phosphorylated p38 were determined at least three times, and the representative data are shown. (B) Cells were stimulated by 500 ng/ml LPS for 6 h and the protein was collected. Phosphorylated p38 MAPK was immunoprecipitated. The immune complexes were used for testing the effects of DCO-6 and SB203580 on kinase activities. Representative data are shown. Bands from (A–B) were analyzed by densitometry. Quantitative data are shown. *P<0.05 vs control.