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. Author manuscript; available in PMC: 2012 Jun 16.
Published in final edited form as: Oncogene. 2011 Oct 24;31(24):2919–2930. doi: 10.1038/onc.2011.473

Figure 6.

Figure 6

Mouse in vivo model of BMPER knockdown in LLC-2 cells. (a) Triplicates of three murine LLC clones stable expressing either a scrambled shRNA (ScrCont) or one of two different shRNAs targeted against BMPER (mBMPER79 or 80) were incubated for 24 h with BrdU in serum-free medium. Afterwards, the BrdU ELISA was performed. Mean±s.d.; *P<0.01 versus the ScrCont. (b, c) LLC shRNA clones were implanted into the left lung of 8-week old female C57BL/6 mice and tumor growth examined 2 weeks later. (b) Hematoxylin and Eosin (HE)-stained representative cryosections of LLC shRNA clone tumors in mouse lungs. Scale bar, 500 μm. (c) Macroscopic pictures and quantitative analysis of the tumor volume (ScrCont and mBMPER79 n = 9/group, mBMPER80 n = 6/group; mean±s.d.; *P<0.01 versus ScrCont).