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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Mar 2;69(7):506–513. doi: 10.1002/cm.21019

Figure 5. Biochemical analysis of α-synuclein from synaptosome-depleted fractions.

Figure 5

(A) Mouse brains were subjected to sequential fractionation as shown, and the fractions were assayed for synapsin and α-synuclein by Western blotting. Note that synapsin is distributed in both the soluble (S100) and pellet (P100) fractions, whereas α-synuclein is primarily found in the soluble fractions; in agreement with previous studies.

(B) Further sucrose-gradient separation of the S100 shows that both proteins are localized within higher density fractions, suggesting organization into higher-order complexes. Densitometry analysis is shown below.