Skip to main content
. Author manuscript; available in PMC: 2013 Jun 12.
Published in final edited form as: Dev Cell. 2012 May 17;22(6):1208–1220. doi: 10.1016/j.devcel.2012.03.014

Figure 1. Pax3 Binds a Subset of Pax7-Binding Sites.

Figure 1

(A) Overexpression of Pax7- and Pax3-CTAP in primary myoblasts reveals a reduction in endogenous levels of Pax7. Pax3 is not expressed in control cells. Also see Figure S1 for a quantitative estimate of proviral versus endogenous Pax7 protein measured as number of molecules per cell. (B) Analysis of peak locations relative to gene start sites indicates Pax3 and Pax7 binding is predominantly in intergenic or intronic regions. (C) Overlap of Pax3 and Pax7 peak data reveals that Pax3 binds only 6.4% of Pax7 sites. (D) Comparison of peaks subsets for Pax3 and Pax7 indicates a stronger binding of Pax7 to DNA in adult skeletal muscle cells as shown by the MACS (Zhang et al., 2008) scores, calculated as −10 * log10(p-value of the peaks). (E) Plot of Pax3 versus Pax7 reads in common peaks shows that Pax7 peaks have generally higher sequence reads than Pax3 counterparts. Counts in the legend indicate number of peaks.