Figure 6. ECR111 is an Enhancer Element Required for Myf5 Expression in Quiescent Satellite Cells.
(A–H) Cryosections of adult Soleus (A,B,E,F) and EDL (C,D,G,H) muscles from B195APZWT (A–D) and B195APZΔ111 (E–H) transgenic lines show that in the absence of the ECR111 enhancer harboring the Pax7 binding site, expression in the satellite cells is abolished. Note that in transgenic animals carrying the deleted BAC construct expression in muscle spindle cells, under the transcriptional control of a separate enhancer, is not affected. Red boxes in A, C, E and G indicates the zoomed regions shown in B, D, F and H, respectively. Additional satellite and muscle spindle cells not included within the close-up figures are also indicated (black and white arrows, respectively).
