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. Author manuscript; available in PMC: 2013 Jul 7.
Published in final edited form as: Nanoscale. 2012 May 29;4(13):3843–3851. doi: 10.1039/c2nr30467h

Figure 1.

Figure 1

Experimental design for in vitro studies described here. A cysteine-appended epitope is integrated into the protecting organic shell of a hydrophilic monolayer-protected AuNP by ligand exchange. The gold electrode on a QCM chip is functionalized with a protein matrix (1) designed to immobilize the selected antibody while resisting non-specific binding to the biomimic. The antibody (2) is added, and subsequently the biomimetic AuNP (3) is brought into contact with the chip. After each addition, the chip is washed to ensure the elimination of non-specifically bound material. Observed mass increase in the final step that is not washed away is indicative of immunorecognition via biomimic-antibody binding.