Figure 4.

Detection of OVA-specific T cells in PVR lesions at 48 and 72 h time point. OVA-specific, OT II TCR-transgenic T cells were labeled with CFSE and injected i.v. into congenic Rag-1 KO mice that received a simultaneous intraocular dispase injection. The mice were killed 12, 24, 48, 72, and 96 h later and cryosections of their eyes were studied for the presence of green-fluorescent (CFSE positive) cells. Hoechst 33342 dye was used to stain nuclei of the tissue blue. Anti-CD3 staining is show in red.