Figure 3.

Aquaporin-4 (AQP4) deficiency downregulates glutamate transporter-1 (GLT-1) expression without altering GLAST level in the lateral amygdala (LA). (a) Representative images of immunoblots using antibodies against GLAST-1 and β-actin. (b) Representative images of immunoblots using antibodies against GLT-1 and β-actin. (c) Quantitative analyses of GLAST by densitometry are summarized. Data were expressed as a percentage of value of wild-type (WT). No significant differences in GLAST expression were observed between AQP4 knockout (KO) and WT mice. (d) Quantitative analyses of GLT-1 by densitometry are summarized. Data were expressed as percentage of value of WT. The expression of GLT-1 was dramatically reduced in AQP4 KO mice compared with those in WT mice (n=6, ^**P<0.01 vs WT).