Figure 1.

Candida albicans biofilm killing by TTO, T-4-ol, and α-terpineol is time dependent. Standardized C. albicans (1 × 10⁶ cells/ml) were incubated in flat-bottomed 96-well plates for 24 h, washed in PBS, and treated with 2 × SMIC₅₀ at 2, 5, 10, 15, and 60 min. Metabolic activity of treated biofilms was then quantified using the XTT assay by reading at 492 nm in a microtiter plate reader (FluoStar Omega, BMG Labtech). Three isolates were used for each assay, and this was performed on two independent occasions in triplicate. **p < 0.001. Error bars represent the ±standard error of the mean.