Figure 2.

Candida albicans biofilm development is inhibited by TTO, T-4-ol, and α-terpineol. Standardized C. albicans (1 × 10⁶ cells/ml) were added to a flat-bottomed 96-well plate and treated with (A) 0.5 and (B) 1 × SMIC₅₀ for 0, 1, 2, and 4 h post incubation. Biofilms were subsequently allowed to develop for 24 h. Resultant biofilms were quantified using a crystal violet assay read at 570 nm in a microtiter plate reader (FluoStar Omega, BMG Labtech). Four isolates were used for each assay, and this was performed on two independent occasions in triplicate. *p < 0.05. Error bars represent the ±standard error of the mean.