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. 2011 Oct 28;21(10):1726–1735. doi: 10.1089/scd.2011.0411

FIG. 2.

FIG. 2.

Osteogenic phenotype of mouse embryoid bodies. Embryoid bodies were cultured for 14 days in medium or medium supplemented with BGP. EBs were then treated with 0.01% ethanol or 1α,25(OH)2D3 for 24 h. DNA content (A) and alkaline phosphatase specific activity (B) were measured in cell lysates. Secreted osteocalcin (C), osteoprotegerin (D), and osteopontin (E) were measured in the conditioned medium. *P<0.05, versus control; #P<0.05, versus 1α,25(OH)2D3. To assess rapid, membrane-initiated signaling, protein kinase C activity was measured in EBs treated with 0.01% ethanol or 1α,25(OH)2D3 for 9 min (F). *P<0.05, versus control; #P<0.05, versus 1α,25(OH)2D3; $P<0.05, versus BGP. 1α,25(OH)2D3,1α,25-dihydroxyvitaminD3.