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Osteogenic phenotype of mouse embryoid bodies. Embryoid bodies were cultured for 14 days in medium or medium supplemented with BGP. EBs were then treated with 0.01% ethanol or 1α,25(OH)2D3 for 12 h. Expression of Tnfsf11 (A), osteopontin (Opn, B), Itga2 (C), and Itgb1 (D) were measured by real-time qPCR. Gene expression was normalized to expression of Gapdh mRNA per 1 ug RNA. *P<0.05, versus control; #P<0.05, versus 1α,25(OH)2D3.
