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. 2011 Sep 20;21(10):1779–1793. doi: 10.1089/scd.2011.0424

FIG. 5.

FIG. 5.

MEK/ERK inhibitor affects c-Myc expression, c-Myc-Max inhibitor; 10074-G5 inhibits pERK expression. (A) Cell lysates from U251 and 5310 cells either untreated or treated with 10 μM U0126 for 12 h were analyzed by immunoblotting with specific antibodies for indicated proteins. β-actin expression shows the equal loading of samples. (B) Semi-quantitative RT-PCR analysis was carried out to detect ERK and c-Myc in U0126 treatments. β-actin expression was used as a loading control. (C, D) U251 and 5310 cells were treated with 25 μM 10074-G5 for 24 h. Immunoblot of total lysates was performed using antibodies specific for the indicated proteins. Fold decrease over the control was obtained by RT-PCR analysis. (E) Eight hundred microgram of total soluble protein from U251 and 5310 control and hUCBSC-treated tissue lysates were immunoprecipitated with pERK, and the samples were immunoblotted against c-Myc. The same blots were stripped and re-probed with anti-pERK antibody. (F) Immunoblot showing the expression levels of Mad and Max in the coculture treatments and compared with control U251 and 5310 cells.